Perhaps the most important and most commonly used in medical practice hormonal drug is insulin, Somatropin. Human insulin – a hormone synthesized by pancreatic beta cells – play a huge role in the normal functioning of the human body’s processes.
The most important feature is its – ensuring the body’s main energy cell material – glucose. Buy Somatropin.
If not enough insulin, cells can not metabolize glucose, it accumulates in the blood and tissues and organs experience energy starvation. With a lack of insulin develops a serious disease such as diabetes.
Prior to the beginning of XX century. diabetics die in childhood or young ages of the various complications of the illness, Somatropin for sale, almost nobody has been able to live more than 5-7 years after the onset of the disease.
The role of the pancreas in the development of diabetes has become known only at the end of the XIX century. In 1869 in Berlin, 22-year-old medical student Paul Langerhans, studying with a microscope the structure of the pancreas, called attention to the previously unknown cell, forming groups that were evenly distributed throughout the gland, Somatropin order, but the function of these cells, called islets of Langerhans then , remained unknown.
Later Ernst Lako hypothesized that the pancreas is involved in the processes of digestion. In 1889 German physiologist Oskar Minkowski attempted to prove that the value of the pancreas in digestion far-fetched. To do this, he put an experiment in which made the removal of the prostate in a healthy dog. A few days after the start of the experiment assistant Minkowski, monitor the condition of laboratory animals, drew attention to the large number of flies that flew in the urine of experimental dogs. Buy Somatropin online.
After the urine he found the dog, devoid of the pancreas, the sugar excreted in the urine. This was the first observation, which tied the pancreas and the development of diabetes. In 1901, Eugene Opie proved that diabetes is caused by abnormalities in the structure of the pancreas, namely the complete or partial destruction of the islets of Langerhans, legal Somatropin.
The first who was able to identify insulin and use it successfully for the treatment of patients, was a Canadian physiologist Frederick Banting. To try to create a cure for diabetes of the young scientist pushed tragic events – two of his friends have died from diabetes hgh peptides. Before Bunting many researchers, understanding the role of the pancreas in developing diabetes, trying to identify the substance, which would directly affect the level of blood sugar, but all attempts ended in failure. Buy Human Growth Hormone.
These failures were caused including that of pancreatic enzymes (trypsin mainly) time to at least partially decompose insulin protein molecules before they could be isolated from prostate tissue extract. In 1906 Georg Ludwig Zeltseru managed to achieve some success in reducing the level of glucose in the blood of experimental dogs using pancreatic extract, legal Human Growth Hormone, but he was unable to continue his work. Scott in 1911, the University of Chicago used aqueous pancreatic extracts and noted a slight decrease in glycosuria in experimental animals, but he was unable to convince his boss to the importance of their research, and these experiments were soon discontinued.
The same effect showed Kleiner and Israel in 1919, but did not complete the work due to the outbreak of the First World War. Buy Human Growth Hormone online.
A similar operation in 1921 and published a professor of physiology at the Romanian School of Medicine Nikola Paulesko, and many, including in Romania, it is considered the discoverer of insulin. However, the merit of insulin and its successful application belongs to Frederick Banting.
Bunting worked as a junior lecturer at the Department of Anatomy and Physiology in one of the Canadian universities, led by Professor John Macleod, was then considered a great expert on diabetes. Bunting tried to get pancreatic atrophy using ligation its excretory ducts (channels) for 6-8 weeks, while keeping unchanged the islets of Langerhans from the effects of pancreatic enzymes, and get a pure extract of cells of the islets, Human Growth Hormone for sale.
For this experiment required laboratory assistants and experimental dogs, whom Bunting was not.
For help, he turned to Professor John McLeod, who knew well about the previous failure to obtain the pancreatic hormones. At first he did not allow Banting in his laboratory. However, Banting did not depart from the spring of 1921 and again asked McLeod to allow work in the lab at least two months. Since that time, McLeod was going to go to Europe and the laboratory was free, he agreed. As an assistant Bunting was given to a student of the 5th year, Human Growth Hormone order, Charles Best, who has studied methods for determining blood sugar and urine.
For the experiment, which required large expenditures, Bunting had to sell almost all of their possessions.
Several dogs were tied up ducts of the pancreas, and then wait for it atrophy. July 27, 1921 dog with remote pancreas, located in precoma, introduced extract atrophied pancreas. A few hours later the dog showed a decrease in blood sugar levels and urine disappeared acetone, Human Growth Hormone.
Then pancreas extract has been entered for the second time and it lived another 7 days. Maybe a dog would have lived longer, but researchers have run out of extract, since getting the insulin from the pancreas glands of dogs was extremely laborious and time-consuming work.
Subsequently, Banting and Best started getting extracts from the pancreas of unborn calves, who have not yet worked out digestive enzymes, but synthesize sufficient amounts of insulin. The amounts of insulin are now enough for it to sustain life experimental dogs have up to 70 days. McLeod, who returned at that time in Europe, gradually became interested in the work of Banting and Best, and connect to it the entire staff of the laboratory. Bunting, who was originally named derived pancreatic extract isletinom, at the suggestion of McLeod renamed it into insulin (from the Latin insu-1a -. “Island”).